Reaction #2146681

Reaction equation

sodium phosphate dibasic

sodium phosphate monobasic

sodium phosphate monobasic

sodium phosphate dibasic

sodium phosphate monobasic

phosphate

→

sodium phosphate dibasic

sodium phosphate

Solvents

water

water

Conditions

Workup

1. 1
   workup.ADDITIONThe stock buffer was diluted as appropriate
2. 2
   Concentrationconcentration and salt
3. 3
   workup.ADDITIONwas added as necessary
4. 4
   workup.ADDITIONcontaining 35 mM NaCl
5. 5
   workup.ADDITIONby mixing 100 ml 0.5 M Sodium phosphate buffer, pH 6.5 and 7 ml 5 M NaCl in water to a final volume of 1 L
6. 6
   FiltrationThe buffer was filtered
7. 7
   Otherdegassed
8. 8
   workup.ADDITIONcontaining 10 mM NaCl
9. 9
   workup.ADDITIONby mixing 100 ml sodium phosphate buffer, pH 7.4 and 2 ml 5 M NaCl in water to a final volume of 1 L
10. 10
    FiltrationThe buffer was filtered
11. 11
    Otherdegassed

Procedure

Stock solutions for phosphate buffers were prepared as follows: 0.5 M solution of sodium phosphate monobasic (FW=119.96) was made by dissolving 59.98 g sodium phosphate monobasic in a final volume of 1 L of water. 0.5 M solution of sodium phosphate dibasic (FW=141.96) was prepared by dissolving 70.98 g sodium phosphate dibasic in a final volume of 1 L of water. A stock of 0.5 M sodium phosphate buffer was prepared by mixing 0.5 M sodium phosphate monobasic and 0.5 M sodium phosphate dibasic while stiffing and monitoring until the desired pH was reached. The stock buffer was diluted as appropriate to reach the desired concentration and salt was added as necessary to reach the desired conductivity. Sodium phosphate buffer, pH 6.5 is a 50 mM sodium phosphate buffer, pH 6.5 containing 35 mM NaCl made by mixing 100 ml 0.5 M Sodium phosphate buffer, pH 6.5 and 7 ml 5 M NaCl in water to a final volume of 1 L. The buffer was filtered, degassed and the conductivity was measured before use. Conductivity of the buffer was recorded as ˜8 mS/cm. Sodium phosphate, pH 7.4 is a 50 mM sodium phosphate buffer, pH 7.4 containing 10 mM NaCl was made by mixing 100 ml sodium phosphate buffer, pH 7.4 and 2 ml 5 M NaCl in water to a final volume of 1 L. The buffer was filtered, degassed and the conductivity was measured before use. Conductivity of the buffer was recorded as: ˜7.2 mS/cm.

Source