Reaction #2041

ord-48e5492592614940b5cbab975adec0bb

Reaction equation

[Ca+2].[Cl-].[Cl-]
calcium chloride
O=P([O-])([O-])OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Na+]
sodium pyrophosphate
[Ca].[P]
calcium phosphorus
→
O.O.O=P([O-])([O-])OP(=O)([O-])[O-].[Ca+2].[Ca+2]
calcium diphosphate dihydrate

Reactants

[Ca+2].[Cl-].[Cl-]
calcium chloride
O=P([O-])([O-])OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Na+]
sodium pyrophosphate
[Ca].[P]
calcium phosphorus

Reagents

None

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Workup

  1. 1
    workup.WAITto stand for a whole day

Procedure

An aqueous calcium chloride solution and an aqueous sodium pyrophosphate solution were mixed together so that the calcium/phosphorus atomic ratio became 1:1, and the mixture was allowed to stand for a whole day to form calcium diphosphate dihydrate (Ca2P2O7.2H2O) crystals, which were then filtered off. The thus prepared material was packed into a column in the same manner as in Example 1, and a mixed sample of serum albumin, lysozyme and cytochrome c was chromatographed thereon. As a result the serum albumin flowed through the column without being retained therein, while the lysozyme and cytochrome c were retained therein.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US05728463uspto-grants-1998_03