Reaction #1973951

ord-9924fa7670674349945a1f7c086af5a4

Reaction equation

CN1CCCC1=O.CO.OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O
X-gal NMP methanol
→
CN1CCCC1=O.CO
NMP Methanol

Reactants

CN1CCCC1=O.CO.OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O
X-gal NMP methanol

Reagents

None

Solvents

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O
X-gal

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Procedure

When the 1× X-gal/NMP/methanol is stored for 16 weeks at 4° C., there is no loss in X-gal activity as measured by applying 100 μl to a LB plate, streaking E. Coli pUC19 transfectant, incubation at 37° C. and examination for blue colonies after 16 hours.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US08501433B2uspto-grants-2013_08