Reaction #1771999

Reaction equation

MBHA resin

Fmoc-Phg-OH

→

ninhydrin

H-Phg MBHA Resin

Solvents

Conditions

Workup

1. 1
   Otherequipped with a nitrogen inlet
2. 2
   WashIt was then washed thoroughly with 30 ml portions of dichloromethane, methanol, dimethylformamide and dichloromethane

Procedure

The commercially available MBHA resin (2.6 g, 1.12 mmol/g, 2.91 mmol) was transferred to a 250 mL fritted solid phase reaction vessel equipped with a nitrogen inlet. It was then washed thoroughly with 30 ml portions of dichloromethane, methanol, dimethylformamide and dichloromethane and coupled over 18 hours to the commercially available Fmoc-Phg-OH (2.17 g, 5.82 mmol) according Procedure A with 99.82% efficiency. The resin was then subjected to Fmoc deprotection according to procedure B. A qualitative ninhydrin assay on a small aliquot gave dark blue resin and solution, indicating a successful reaction.

Source