Reaction #1747852

Reaction equation

4-carboxyphenylthiourea

DIC

→

Phenylthiourea

Solvents

DMF

Conditions

Workup

1. 1
   OtherAminopropylsilylated glass surfaces purified with compressed air (2.5×7.5 cm; Sigma, Silane-Prep™, S4651)
2. 2
   Otherthus obtained
3. 3
   workup.ADDITIONThe glass surfaces thus treated
4. 4
   Washwere then washed five times
5. 5
   Otherfor 3 minutes
6. 6
   Otherat room temperature
7. 7
   WashAfter washing three times for three minutes each using respectively 30 mL of DCM at room temperature
8. 8
   Otherthe glass surfaces were dried
9. 9
   Otherstored at 4° C. until further use

Procedure

4-carboxyphenylthiourea (Lancaster, 13047) was dissolved to 0.2M in DMF. The resultant mixture was activated by adding a half equivalent of DIC for 15 min at room temperature. Aminopropylsilylated glass surfaces purified with compressed air (2.5×7.5 cm; Sigma, Silane-Prep™, S4651) were coated with the activated solution thus obtained and incubated for three hours at room temperature. The glass surfaces thus treated were then washed five times using respectively 30 mL of DMF for 3 minutes each at room temperature. After washing three times for three minutes each using respectively 30 mL of DCM at room temperature, the glass surfaces were dried and stored at 4° C. until further use.

Source