Reaction #1596981

ord-36e9f754a31f4801b2a657445eb30933

Reaction equation

CCNC(=O)CC[C@H](N)C(=O)O
theanine
N[C@@H](CCC(=O)O)C(=O)O
glutamic acid
CCNC(=O)CC[C@H](N)C(=O)O
theanine
→
CCNC(=O)CC[C@H](N)C(=O)O
theanine
N[C@@H](CCC(=O)O)C(=O)O
glutamic acid
NC(=O)CC[C@H](N)C(=O)O
glutamine

Reactants

CCNC(=O)CC[C@H](N)C(=O)O
theanine
N[C@@H](CCC(=O)O)C(=O)O
glutamic acid
CCNC(=O)CC[C@H](N)C(=O)O
theanine

Reagents

None

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Workup

  1. 1
    Othersynthesis
  2. 2
    workup.ADDITIONwas executed was diluted suitably

Procedure

An enzyme reaction liquid in which theanine enzyme synthesis was executed was diluted suitably and thereafter, HPLC was carried out for the diluted reaction liquid so that amounts of theanine and glutamic acid were determined. A mol conversion ratio from an amount of glutamine (mol/L) of the substrate was calculated using obtained amounts of theanine and glutamic acid (mol/L). TABLE 1 shows the conditions for determination by HPLC.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US08211674B2uspto-grants-2012_07