Reaction #1552060

Reaction equation

tris(hydroxymethyl)aminomethane

hydrochloric acid

→

Tris-HCl

Solvents

water

Conditions

Procedure

Herein, 6.057 g of tris(hydroxymethyl)aminomethane (by Nacalai Tesque) were dissolved in pure water, and thereafter the pH was adjusted to pH 7.5 with hydrochloric acid (by Nacalai Tesque), to yield a 50 mL solution (hereafter, Tris-HCl buffer solution). Further, 1.06 mg of β-NADH (by Oriental Yeast), 7.5 mg of sucrose (by Nacalai Tesque) as a saccharide, and 0.25 mg of 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) (by Dojindo Laboratories), as a surfactant, were dissolved in 0.238 mL of pure water, followed by addition of 0.013 mL of the Tris-HCl buffer solution, with agitation, to prepare a reagent solution. In this case, the concentration of sucrose in the reagent solution is 3 W/V %, and the CHAPS concentration is 0.1 W/V %. The present example differs from Example 1 to Example 6 in that herein β-NADH is used, instead of β-NAD+, as the nicotinamide coenzyme.

Source