Reaction #1447080

ord-af08bfc8067f41a89e53558cd6400aad

Reaction equation

O=C(O[C@@H]1Cc2c(O)cc(O)cc2O[C@@H]1c1cc(O)c(O)c(O)c1)c1cc(O)c(O)c(O)c1
EGCG
C[S+](CC[C@H](N)C(=O)O)C[C@H]1O[C@@H](n2cnc3c(N)ncnc32)[C@H](O)[C@@H]1O
S-adenosyl-L-methionine
→
Oc1cc(O)c2c(c1)OC(c1ccc(O)c(O)c1)C(O)C2
catechin

Reactants

O=C(O[C@@H]1Cc2c(O)cc(O)cc2O[C@@H]1c1cc(O)c(O)c(O)c1)c1cc(O)c(O)c(O)c1
EGCG
C[S+](CC[C@H](N)C(=O)O)C[C@H]1O[C@@H](n2cnc3c(N)ncnc32)[C@H](O)[C@@H]1O
S-adenosyl-L-methionine

Reagents

None

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Workup

  1. 1
    Otherto proceed at 5 to 60° C., preferably at 20 to 40° C.

Procedure

The purified enzyme is suspended in a buffer having a pH of 4.5 to 8.5, preferably 6.5 to 8. To this suspension, EGCG or ECG is added along with S-adenosyl-L-methionine (SAM) and the reaction is allowed to proceed at 5 to 60° C., preferably at 20 to 40° C., to form a methylated catechin.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US07879586B2uspto-grants-2011_02