Reaction #1442501

ord-d186d3bfad114737971cb7092f27d2f3

Reaction equation

NCCc1ccc(O)c(O)c1
dopamine
N[C@@H](Cc1ccc(O)c(O)c1)C(=O)O
dopa
NCCc1ccc(O)c(O)c1
dopamine
→
NCCc1ccc(O)c(O)c1
dopamine
N[C@@H](Cc1ccccc1)C(=O)O
Phe

Reactants

NCCc1ccc(O)c(O)c1
dopamine
—
trinitrophenyl-1-dopamine
N[C@@H](Cc1ccc(O)c(O)c1)C(=O)O
dopa
NCCc1ccc(O)c(O)c1
dopamine

Reagents

None

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Procedure

The formation of trinitrophenyl-1-dopamine from dopamine (and hence the formation of dopamine from dopa) was measured in a spectrophotometer using ε340 nm=12,400 M−1 cm−1. In this assay, Phe-DC formed 9.4 nmol of dopamine and Di-ADC formed 2.74 nmol of dopamine, demonstrating once again that Di-ADC is an aromatic amino acid decarboxylase.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US06586173B2uspto-grants-2003_07