Reaction #1435681

Reaction equation

DIEA

Fmoc-L-proline pentafluorophenyl ester

Gly-L-Phe-L-Leu

→

L-Pro-Gly-L-Phe-L-Leu

Solvents

DMF

Conditions

Workup

1. 1
   Washwashed with DMF (4×) and methylene chloride (2×)
2. 2
   workup.WAITDeprotection can be effected by treatment with a solution of 20% piperidine in DMF for 30 min
3. 3
   WashThe ELAMS™ are then washed with DMF (2×), ethanol (2×) and methylene chloride (2×)
4. 4
   Otherdried at 60° C. for 4 hours

Procedure

Fmoc-L-proline pentafluorophenyl ester (50 mg, 0.1 mmol) is dissolved in anhydrous DMF (1 mL) containing DIEA (17 μL, 0.1 mmol). About 0.5-1.0 mL of this solution is added to Gly-L-Phe-L-Leu ELAMS™ in a test tube and the tube vortexed for about 3 hours. The ELAMS™ are pelleted and washed with DMF (4×) and methylene chloride (2×). Deprotection can be effected by treatment with a solution of 20% piperidine in DMF for 30 min. The ELAMS™ are then washed with DMF (2×), ethanol (2×) and methylene chloride (2×) and dried at 60° C. for 4 hours. The identifier tag for each ELAMS™ is detected and recorded.

Source