Reaction #1379911

ord-3f965294fdba46499b3700bfb8d4fe93

Reaction equation

O=S(=O)(O)CCN1CCOCC1
MES
O=C(O)[C@@H]1CCCN1
L-proline
O=C([O-])CCC(=O)C(=O)[O-]
2-ketoglutarate
O=C1O[C@H]([C@@H](O)CO)C(O)=C1O
L-ascorbic acid
→
O=C(O)[C@@H]1C[C@@H](O)CN1
trans-4-hydroxy-L-proline

Reactants

O=S(=O)(O)CCN1CCOCC1
MES
O=C(O)[C@@H]1CCCN1
L-proline
O=C([O-])CCC(=O)C(=O)[O-]
2-ketoglutarate
O=C1O[C@H]([C@@H](O)CO)C(O)=C1O
L-ascorbic acid
—
ferrous sulfates
—
L-proline-4

Reagents

None

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Workup

  1. 1
    Otherobtained in Example 5
  2. 2
    OtherAs a result of the reaction

Procedure

The enzyme reaction with purified L-proline-4-hydroxylase obtained in Example 5 was carried out. The reaction mixture was composed of 200 mM MES buffer (pH 6.5), 20 mM L-proline, 20 mM 2-ketoglutarate, 5 mM L-ascorbic acid, 2 mM ferrous sulfates and 90 U of purified enzyme preparation in total volume of 50 μl. The reaction was carried out at 35° C. for 30 min. As a result of the reaction, 12.9 mM (1.7 g/l) of trans-4-hydroxy-L-proline was produced in the reaction mixture.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US07238501B2uspto-grants-2007_07