Reaction #1379908

ord-676857194c084ad08b76505f5b3d1379

Reaction equation

O=C(O)[C@@H]1CCCN1
L-proline
O=C(O)CCC(=O)C(=O)O
2-ketoglutaric acid
O=C1O[C@H]([C@@H](O)CO)C(O)=C1O
L-ascorbic acid
O=S(=O)(O)CCN1CCOCC1
MES
→
O=C(O)[C@@H]1C[C@@H](O)CN1
trans-4-hydroxy-L-proline

Reactants

O=C(O)[C@@H]1CCCN1
L-proline
O=C(O)CCC(=O)C(=O)O
2-ketoglutaric acid
O=C1O[C@H]([C@@H](O)CO)C(O)=C1O
L-ascorbic acid
—
ferrous sulfate
O=S(=O)(O)CCN1CCOCC1
MES

Reagents

None

Conditions

Temperature
100°CELSIUS
Detailed conditions
See reaction.notes.procedure_details.

Workup

  1. 1
    OtherThe cells, the treated cells or the enzyme preparation
  2. 2
    Otherthe reaction

Procedure

The cells, the treated cells or the enzyme preparation are added to 240 mM MES [2-(N-morphorino) ethanesulfonic acid] buffer containing 12 mM L-proline, 24 mM 2-ketoglutaric acid, 4 mM ferrous sulfate and 8 mM L-ascorbic acid to make 250 μl in total. The mixture is kept at 35° C. for 10 minutes. The reaction mixture is heated at 100° C. for 2 minutes to stop the reaction, and the amount of trans-4-hydroxy-L-proline produced in the reaction mixture is determined by HPLC.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US07238501B2uspto-grants-2007_07