Reaction #1355848

ord-58ceeb54159540d89c15caf417f52855

Reaction equation

O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-]
citrate
C=C(OP(=O)(O)O)C(=O)O
phosphoenolpyruvate
N[C@@H](CCC(=O)[O-])C(=O)[O-]
glutamate
→
N[C@@H](CCC(=O)O)C(=O)O
L-glutamic Acid

Reactants

O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-]
citrate
C=C(OP(=O)(O)O)C(=O)O
phosphoenolpyruvate
N[C@@H](CCC(=O)[O-])C(=O)[O-]
glutamate

Reagents

None

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Workup

  1. 1
    workup.ADDITIONwere introduced into the SC17sucA strain

Procedure

Subsequently, the citrate synthase gene, phosphoenolpyruvate carboxylase gene and glutamate dehydrogenase gene derived from Escherichia coli were introduced into the SC17sucA strain.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US07198943B2uspto-grants-2007_04