Reaction #1244260

ord-b0b8846a234f4d108aa157a59f0703e9

Reaction equation

Nc1nc2nc[nH]c2c(=O)[nH]1.O=P(O)(O)O
guanine monophosphate
Nc1nc(=O)c2ncn([C@@H]3O[C@@H]4COP(=O)(O)O[C@H]4[C@H]3O)c2[nH]1
cGMP
Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
ATP
→
Nc1nc2c(ncn2[C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]2O)c(=O)[nH]1
guanosine-5′-diphosphate
Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
ADP

Reactants

Nc1nc2nc[nH]c2c(=O)[nH]1.O=P(O)(O)O
guanine monophosphate
Nc1nc(=O)c2ncn([C@@H]3O[C@@H]4COP(=O)(O)O[C@H]4[C@H]3O)c2[nH]1
cGMP
Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
ATP

Reagents

None

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Procedure

The cGMP present in the sample is then converted to GMP with phosphodiesterase. The GMP is combined with ATP in the presence of guanine monophosphate kinase to yield guanosine-5′-diphosphate (GDP) and ADP.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US07807367B2uspto-grants-2010_10