Reaction #1244242

ord-a8157e506ba647088ae91dc2f0088eb6

Reaction equation

O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-]
citrate
C=C(OP(=O)(O)O)C(=O)O
phosphoenolpyruvate
N[C@@H](CCC(=O)[O-])C(=O)[O-]
glutamate
→
N[C@@H](CCC(=O)O)C(=O)O
L-glutamic Acid

Reactants

O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-]
citrate
C=C(OP(=O)(O)O)C(=O)O
phosphoenolpyruvate
N[C@@H](CCC(=O)[O-])C(=O)[O-]
glutamate

Reagents

None

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Workup

  1. 1
    workup.ADDITIONwere introduced into the SC17sucA strain

Procedure

Subsequently, the citrate synthase gene, phosphoenolpyruvate carboxylase gene and glutamate dehydrogenase gene derived from Escherichia coli were introduced into the SC17sucA strain.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: USRE041800E1uspto-grants-2010_10