Reaction #1095843

ord-0b8817fba5b1428fa0d04d7da66ed1ec

Reaction equation

CN(C)[C@@H]1C(O)=C(C(N)=O)C(=O)[C@@]2(O)C(O)=C3C(=O)c4c(O)cccc4[C@@](C)(O)[C@H]3C[C@@H]12
tetracycline
CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O
ampicillin
→
C[C@H](O)[C@H](O)[C@@H](O)[C@@H](O)C=O
L-Rhamnose

Reactants

CN(C)[C@@H]1C(O)=C(C(N)=O)C(=O)[C@@]2(O)C(O)=C3C(=O)c4c(O)cccc4[C@@](C)(O)[C@H]3C[C@@H]12
tetracycline
CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O
ampicillin

Reagents

None

Conditions

Detailed conditions
See reaction.notes.procedure_details.

Procedure

The plasmid pBRsaCH3 (Example 1) was used for cloning the rMET1 antibody (Example 9). In this case, the kappa and heavy chains were engineered with human ceruloplasmin and human neutrophil defensin signal sequences, respectively (Example 9). This plasmid was electroporated into E. coli strain DH10B rha+ to generate clone rMET1.LS. 1, which was cultured under 10 μg/mL tetracycline selection. The plasmid was also electroporated into E. coli strain ECL339 rha− to generate clone rMET1.ECL. 1 which was cultured under 100 μg/mL ampicillin selection.

Source

DOI: 10.6084/m9.figshare.5104873.v1Patent: US07972811B2uspto-grants-2011_07