Reaktion #96221

ord-80c7c28f366447698cd962b139a4b19a

Reaktionsgleichung

Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
ADP
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
→
Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
ATP

Edukte

—
MgCl2.6H2O
Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
ADP
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose

Reagenzien

Keine

Lösungsmittel

O
water

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    SonstigeThe resting cells mentioned above were dried at a room temperature
  2. 2
    Sonstigedried further in a phosphorus pentoxide desiccator under a vacuum overnight
  3. 3
    Temperaturheated
  4. 4
    Sonstigefor 3 minutes

Vorschrift

The resting cells mentioned above were dried at a room temperature and dried further in a phosphorus pentoxide desiccator under a vacuum overnight. Fifty mg of the dried cells were put into 2 ml of a reaction mixture containing 10 μmoles ADP, 200 μmoles glucose 250 μmoles phosphate buffer, 5 μmoles MgCl2.6H2O and the amount of the inhibitor shown in Table 5 or 6. The reaction mixture was held at 30° C. for 3 hours, and then heated in boiling water for 3 minutes. ATP formed in the reaction mixture was determined colorimetrically.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US04347317uspto-grants-1982_08