Reaktion #940970

ord-5cd557340ebd447090a4e7284c94d7c7

Reaktionsgleichung

CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](NC(=N)N)[C@@H](O)[C@@H]3NC(=N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O
streptomycin
CC1(C)S[C@@H]2[C@H](NC(=O)Cc3ccccc3)C(=O)N2[C@H]1C(=O)[O-].[K+]
penicillin
O=C=O
carbon dioxide
→
CN1CC[C@]23c4c5ccc(O)c4O[C@H]2[C@@H](O)C=C[C@H]3[C@H]1C5
Morphine

Edukte

CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](NC(=N)N)[C@@H](O)[C@@H]3NC(=N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O
streptomycin
CC1(C)S[C@@H]2[C@H](NC(=O)Cc3ccccc3)C(=O)N2[C@H]1C(=O)[O-].[K+]
penicillin
O=C=O
carbon dioxide

Reagenzien

Keine

Reaktionsbedingungen

Temperatur
37°CELSIUS
Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    TemperaturHuman neuroblastoma SH-SY5Y cells were maintained as a monolayer culture in Dulbecco's
  2. 2
    workup.WAITCells were plated into flasks a week before experiments Treatments
  3. 3
    Sonstigewere terminated at the designated times
  4. 4
    Waschensubsequently rinsed with PBS three times

Vorschrift

Human neuroblastoma SH-SY5Y cells were maintained as a monolayer culture in Dulbecco's modified eagle medium (DMEM) supplemented with 10% fetal calf serum, 100 μg/ml streptomycin and 100 units/ml penicillin in 5% carbon dioxide, incubator maintained at 37° C. (Wang et al., 2000). Cells were plated into flasks a week before experiments Treatments were terminated at the designated times by replacing the medium with ice-cold phosphate buffered saline (PBS) on ice and subsequently rinsed with PBS three times. Cells then were used for cAMP assay or harvested as described in Section C10

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US07256200B2uspto-grants-2007_08