Reaktion #934384

Reaktionsgleichung

p-nitrobenzyl 2-(4-methyl-1,2,3-triazol-1-yl)-carbapen-2-em-3carboxylate

p-nitrobenzyl 2-(5-methyl-1,2,3-triazol-1-yl)-carbapen-2-em-3-carboxylate

4-morpholinepropanesulfonic acid

→

2-(5-methyl-1,2,3-triazol-1-yl)-carbapen-2-em-3-carboxylic acid

2-(4-methyl-1,2,3-triazol-1-yl)-carbapen-2-em-3-carboxylic acid

Lösungsmittel

dioxane

water

ethanol

Reaktionsbedingungen

Aufarbeitung

1. 1
   Waschenthe pellet was washed with deionized water (5×0.5 ml)
2. 2
   ExtraktionThe combined supernate and washes were extracted with ethyl acetate (3×0.5 ml)
3. 3
   Einengenconcentrated under vacuum at 0° C.

Vorschrift

A solution of a mixture of p-nitrobenzyl 2-(4-methyl-1,2,3-triazol-1-yl)-carbapen-2-em-3carboxylate and p-nitrobenzyl 2-(5-methyl-1,2,3-triazol-1-yl)-carbapen-2-em-3-carboxylate (0.7 mg) in dioxane (100 microliter), deionized water (60 microliter), absolute ethanol (8.3 microliter), and pH 7.0 0.5M aqueous 4-morpholinepropanesulfonic acid (10.2 microliter) was shaken with 10% (w/w) palladium on charcoal (1.0 mg) under hydrogen (50 psig) at ambient temperature. After 30 min. the mixture was centrifuged and the pellet was washed with deionized water (5×0.5 ml). The combined supernate and washes were extracted with ethyl acetate (3×0.5 ml) and concentrated under vacuum at 0° C. to provide 2.0 ml of a solution of 2-(4-methyl-1,2,3-triazol-1-yl)-carbapen-2-em-3-carboxylic acid and 2-(5-methyl-1,2,3-triazol-1-yl)-carbapen-2-em-3-carboxylic acid, UV λmax 295 nm quenched by addition of hydroxylamine hydrochloride and dipotassium hydrogen phosphate.

Quelle