Reaktion #93039

Reaktionsgleichung

O-Benzyl-D-threonine

4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride

methanol

acetic acid

→

(2R, 3S)-3-Benzyloxy-2-[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonylamino]butyric acid

Lösungsmittel

acetone

water

chloroform

Reaktionsbedingungen

Aufarbeitung

1. 1
   Sonstigegiving a virtually complete solution
2. 2
   workup.ADDITIONwas added dropwise
3. 3
   workup.ADDITIONThe pH was kept at 9.5 by the addition of further base while the solution
4. 4
   Sonstigeto regain room temperature
5. 5
   Sonstigethe bulk of the acetone removed by evaporation
6. 6
   ExtraktionThe oily product was extracted into ethyl acetate (3×50 ml)
7. 7
   Waschenthe total organic phase was washed once with brine
8. 8
   Trocknendried over sodium sulphate
9. 9
   SonstigeEvaporation
10. 10
    Sonstigegave the title acid (1.36 g, 82%) as a colourless non-crystalline foam which

Vorschrift

O-Benzyl-D-threonine (0.92 g, 4.4 mmole) (prepared by the method of T. Mizoguchi, G. Levin, D. W. Woolley, and J. M. Stewart, J. Org. Chem., 1968, 33, 903, who described the L-isomer) was suspended in water (20 ml) and the pH adjusted to 9.5 , giving a virtually complete solution. This was cooled to 0° C. and stirred while 4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride (1.00 g, 4.9 m mole) in AR acetone (10 ml) was added dropwise. The pH was kept at 9.5 by the addition of further base while the solution was allowed to regain room temperature. After 2 h the solution was acidified to pH 2 and the bulk of the acetone removed by evaporation. The oily product was extracted into ethyl acetate (3×50 ml), then the total organic phase was washed once with brine and dried over sodium sulphate. Evaporation gave the title acid (1.36 g, 82%) as a colourless non-crystalline foam which retained solvent traces tenaciously; Rf 0.35 in chloroform:methanol:acetic acid, 17:2:1; δ(CDCl3) 0.75-1.35 (5H, m, CH3CH and NCH2CH3), 3.1-3.7 (4H, m, 2 NCH2), 3.9-4.25 (2H, m, NCH2), 4.50 (2H, m, CH--CH), 6.25 (1H, brs, D2O exch, OH), 7.25 (5H, s, aryl), 9.50 (1H, brd), J 7 Hz, D2O exch, NH), 9.70 (1H, brs, D2O exch, CO2H).

Quelle