Reaktion #92883

Reaktionsgleichung

4'-demethylepipodophyllotoxin-beta-D-glucoside

acetaldehyde-dimethylacetal

p-toluene-sulphonic acid

nitromethane

→

Etoposide

Lösungsmittel

water

chloroform

chloroform

methanol

Reaktionsbedingungen

Aufarbeitung

1. 1
   workup.ADDITIONby diluting with 400 ml
2. 2
   SonstigeThe crude material obtained
3. 3
   Einengenafter concentrating the dried organic phase
4. 4
   Sonstigeby evaporation
5. 5
   Sonstigewas subsequently chromatographed on 100 g
6. 6
   Sonstigewas obtained
7. 7
   SonstigeAfter recrystallization from methanol colorless crystals
8. 8
   Sonstige[α ]D22 =-110.5° (c.=0.588 in chloroform), were obtained

Vorschrift

1.5 g. of dry 4'-demethylepipodophyllotoxin-beta-D-glucoside was suspended in 30 ml. of nitromethane, and 6 ml. of acetaldehyde-dimethylacetal and 150 mg. of p-toluene-sulphonic acid was added. The mixture was stirred at room temperature in an atmosphere of nitrogen and in the absence of moisture for one hour. After this time, the initial suspension had turned into a clear solution, and no starting material could be detected in the thin layer chromatogram (silica gel plates, eluant: chloroform+6% of methanol). Working up was effected by diluting with 400 ml. of chloroform and shaking out three times with 25 ml. each of water. The crude material obtained after concentrating the dried organic phase by evaporation was subsequently chromatographed on 100 g. of silica gel "Merck" (grain size 0.05 to 0.2 mm.), whereby chloroform containing 6% of methanol was continuously used as eluant. 4'-demethylepipodophyllotoxin-beta-D-ethylidene-glucoside, which is uniform according to thin layer chromatography, was obtained. After recrystallization from methanol colorless crystals having a M.P. of 236°-251° C., [α ]D22 =-110.5° (c.=0.588 in chloroform), were obtained.

Quelle