Reaktion #82860

ord-1825b608ebc244d3b221ee45bb66642a

Reaktionsgleichung

$CCCCCCCC/C=C\CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C\CCCCCCCC.[Cl-]$

DOTMA

$CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC$

DOPC

→

$CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC.CCCCCCCC/C=C\CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C\CCCCCCCC.[Cl-]$

DOTMA DOPC

Edukte

$CCCCCCCC/C=C\CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C\CCCCCCCC.[Cl-]$

DOTMA

$CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC$

DOPC

Reagenzien

Keine

Lösungsmittel

chloroform

Reaktionsbedingungen

Detaillierte Bedingungen

See reaction.notes.procedure_details.

Aufarbeitung

1
SonstigeDOTMA/DOPC vesicles were prepared
2
Sonstigeremoving the solvent
3
Sonstigeby drying under a stream of nitrogen gas
4
Sonstigewas placed under vacuum overnight
5
Sonstigeto remove traces of solvent
6
Sonstigesonicated in a bath type sonicator for 30 minutes

Vorschrift

DOTMA/DOPC vesicles were prepared by dissolving 10 mg DOTMA and 10 mg DOPC in chloroform and removing the solvent by drying under a stream of nitrogen gas. This dried film was placed under vacuum overnight to remove traces of solvent. The film was then suspended in 1 ml water with vigorous shaking and sonicated in a bath type sonicator for 30 minutes until clear producing DOTMA/DOPC SUV's at a concentration of 20 mg/ml total lipid.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US05622712uspto-grants-1997_04