Reaktion #74818

Reaktionsgleichung

Nα -(3-Acetylthio-2-methylpropanoyl)-L-arginine

→

Nα -(3-Mercapto-2-methylpropanoyl)-L-arginine

Lösungsmittel

water

ammonia

Reaktionsbedingungen

Aufarbeitung

1. 1
   EinengenAfter one hour at room temperature the solution is concentrated to 3 ml
2. 2
   Temperaturin vacuo (no heat) and ion exchange resin AG-50 W
3. 3
   workup.ADDITIONis added until the pH of approximately 4
4. 4
   Waschenthe Nα -(3-mercapto-2-methylpropanoyl)-L-arginine is eluted with pyridine-acetate buffer pH 6.5
5. 5
   SonstigeThe solvent is removed in vacuo
6. 6
   Sonstigedried

Vorschrift

Nα -(3-Acetylthio-2-methylpropanoyl)-L-arginine (1 g.) is dissolved in a mixture of water (5 ml.) and concentrated ammonia (5 ml.). After one hour at room temperature the solution is concentrated to 3 ml. in vacuo (no heat) and ion exchange resin AG-50 W is added until the pH of approximately 4. The suspension is applied to a column of the same resin and the Nα -(3-mercapto-2-methylpropanoyl)-L-arginine is eluted with pyridine-acetate buffer pH 6.5. The solvent is removed in vacuo and the residue is freeze dried, yield 0.86 g., m.p. 100°.

Quelle