Reaktion #744611

Reaktionsgleichung

AcOH

TFA

N-{[1,6-diethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]methyl}-N′-[(3′-formyl-3-biphenylyl)methyl]propanediamide

1,1-dimethylethyl (2R)-2-methyl-1-piperazinecarboxylate

NaB(OAc)3H

→

title compound

Ausbeute 9.9%

N-{[1,6-Diethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]methyl}-N′-[(3′-{[(3R)-3-methyl-1-piperazinyl]methyl}-3-biphenylyl)methyl]propanediamide

Ausbeute 9.9%

Lösungsmittel

dimethyl sulfoxide

dichloromethane

Reaktionsbedingungen

Aufarbeitung

1. 1
   SonstigeThe reaction was quenched with saturated NaHCO3
2. 2
   Extraktionextracted with EtOAc (3×1 mL)
3. 3
   WaschenCombined organic layers were washed with saturated NaCl
4. 4
   Trocknendried (Na2SO4)
5. 5
   Einengenconcentrated
6. 6
   workup.DISSOLUTIONThe crude products were redissolved in MeOH/DMSO (1/1)
7. 7
   Sonstigepurified
8. 8
   Sonstigesolvent evaporated
9. 9
   Sonstigea GeneVac EZ-2 evaporator
10. 10
    Sonstigethat mixture was placed in a Glas-Col evaporator overnight
11. 11
    workup.DISSOLUTIONthe residue was redissolved in MeOH/DMSO
12. 12
    Sonstigepurified
13. 13
    Sonstigesolvent was evaporated
14. 14
    Sonstigea GeneVac EZ-2 evaporator That residue

Vorschrift

To N-{[1,6-diethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]methyl}-N′-[(3′-formyl-3-biphenylyl)methyl]propanediamide (50 mg, 0.086 mmol) was added 1,1-dimethylethyl (2R)-2-methyl-1-piperazinecarboxylate (34.4 mg, 0.172 mmol) followed by NaB(OAc)3H (36.4 mg, 0.172 mmol) and AcOH (4.91 μL, 0.086 mmol) in dimethyl sulfoxide (DMSO) (0.5 mL). The mixture was stirred overnight at room temperature. The reaction was quenched with saturated NaHCO3 and extracted with EtOAc (3×1 mL). Combined organic layers were washed with saturated NaCl, dried (Na2SO4) and concentrated. The crude products were redissolved in MeOH/DMSO (1/1) and purified using a Gilson HPLC (acidic condition). Product fractions were combined and solvent evaporated using a GeneVac EZ-2 evaporator. To the obtained residue was added TFA (0.198 mL, 2.57 mmol) in dichloromethane (0.2 mL) and that mixture was placed in a Glas-Col evaporator overnight and then the residue was redissolved in MeOH/DMSO and purified using a Gilson HPLC (acidic condition). Product fractions were combined and solvent was evaporated using a GeneVac EZ-2 evaporator That residue was basified by cartridge to afford the title compound 5.7 mg (9.4%). LC-MS m/z 667 (M+H)+.

Quelle