Reaktion #728305

ord-836d6c633ed54cceae662041055e1974

Reaktionsgleichung

O=C[O-]
formate
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
CN(C)[C@@H]1C(O)=C(C(N)=O)C(=O)[C@@]2(O)C(O)=C3C(=O)c4c(O)cccc4[C@@](C)(O)[C@H]3C[C@@H]12
tetracycline
CC(O)C(=O)[O-]
lactate
→
OCCCO
1,3-propanediol
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose

Edukte

O=C[O-]
formate
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
CN(C)[C@@H]1C(O)=C(C(N)=O)C(=O)[C@@]2(O)C(O)=C3C(=O)c4c(O)cccc4[C@@](C)(O)[C@H]3C[C@@H]12
tetracycline
CC(O)C(=O)[O-]
lactate

Reagenzien

Keine

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    workup.ADDITIONThe plasmid pTPG(−) and the control plasmid pTLH1 were introduced into the E

Vorschrift

The plasmid pTPG(−) and the control plasmid pTLH1 were introduced into the E. coli NZN 111 (pfl :cat, Idh :kan) strain, a strain incapable of producing formate and lactate in anaerobiosis. These two recombinant strains were then grown anaerobically for 48 hours in the LB medium+glucose+10 μg/ml of tetracycline without regulation of pH. The results presented in Table 5 show that E. coli can produce significant quantities of 1,3-propanediol from glucose after transformation by the plasmid pTPG.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US07695943B2uspto-grants-2010_04