Reaktion #725709

ord-1d3a85980dba4ee1a0ce9052fe130086

Reaktionsgleichung

[Na+].[OH-]
sodium hydroxide
CC(=O)N[C@@H](CC(C)C)C(=O)O
N-acetyl-leucine
CC[C@H](C)[C@H](NC(C)=O)C(=O)O
N-acetyl-isoleucine
→
CC(C)C[C@H](N)C(=O)O
L-leucine

Edukte

[Na+].[OH-]
sodium hydroxide
—
mixture
CC(=O)N[C@@H](CC(C)C)C(=O)O
N-acetyl-leucine
CC[C@H](C)[C@H](NC(C)=O)C(=O)O
N-acetyl-isoleucine

Reagenzien

Keine

Lösungsmittel

O
water
O
water

Reaktionsbedingungen

Temperatur
36°CELSIUS
Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    workup.ADDITIONThere were added 50 mg of CoCl2X6H2O and 50 mg of commerical pig kidney acylase (1200 U/mg)
  2. 2
    FiltrationBy filtering with suction
  3. 3
    Waschenwashing
  4. 4
    Sonstigedrying there

Vorschrift

86.6 grams of this mixture of equal parts of N-acetyl-leucine and N-acetyl-isoleucine were suspended in 400 ml of water and adjusted to pH 7.0 with 50% aqueous sodium hydroxide. There were added 50 mg of CoCl2X6H2O and 50 mg of commerical pig kidney acylase (1200 U/mg) and the mixture was filled up to 500 ml with water. The clear solution was stirred at 36° C. for 6 hours. By filtering with suction, washing and drying there were obtained 17.9 grams of pure L-leucine having a rotary value of [α]D25 =+15.9°.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US04562153uspto-grants-1985_12