Reaktion #68769

ord-28b9f6fe397143c3afbbfc2187eacc8c

Reaktionsgleichung

COc1ccc(-c2oc3c(CC=C(C)C)c(O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)cc(O)c3c(=O)c2O[C@@H]2O[C@@H](C)[C@H](O)[C@@H](O)[C@H]2O)cc1
icariin
O=P([O-])(O)O.O=P([O-])([O-])O.[Na+].[Na+].[Na+]
sodium dihydrogen phosphate disodium hydrogen phosphate
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
→
COc1ccc(-c2oc3c(CC=C(C)C)c(O)cc(O)c3c(=O)c2O[C@@H]2O[C@H](C)[C@@H](O)[C@H](O)[C@@H]2O)cc1
icariside II

Edukte

COc1ccc(-c2oc3c(CC=C(C)C)c(O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)cc(O)c3c(=O)c2O[C@@H]2O[C@@H](C)[C@H](O)[C@@H](O)[C@H]2O)cc1
icariin
O=P([O-])(O)O.O=P([O-])([O-])O.[Na+].[Na+].[Na+]
sodium dihydrogen phosphate disodium hydrogen phosphate
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose

Reagenzien

Keine

Lösungsmittel

—
β

Reaktionsbedingungen

Temperatur
40°CELSIUS
Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    Sonstigeto remove glucoside
  2. 2
    TemperaturThen stop heating
  3. 3
    Extraktionrepeatedly extract with ethyl acetate
  4. 4
    Sonstigedried
  5. 5
    ExtraktionThen repeatedly extract with methanol

Vorschrift

Fifty grams icariin were added into sodium dihydrogen phosphate-disodium hydrogen phosphate buffer (pH5.5) and 100 ml β glucose. 100 ml of β glucosidase was added and the solution was heated to 40° C. with stirring for 24 hours in order to remove glucoside. Then stop heating and repeatedly extract with ethyl acetate. The ethyl acetate layer was pooled and dried. Then repeatedly extract with methanol. The HPLC analysis confirmed that the content of pure icariside II 50.1%. Thus extract comprising icariside II is obtained.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US08530433B2uspto-grants-2013_09