Reaktion #684943

Reaktionsgleichung

phenylpyruvic acid

sodium formate

NAD

→

(R)-phenyl lactic acid

Lösungsmittel

phosphate

Reaktionsbedingungen

Aufarbeitung

1. 1
   SonstigeAfter completion of the reaction
2. 2
   workup.WAITthe solution was centrifuged at 12,000 rpm for 3 minutes
3. 3
   Sonstigeto remove cells
4. 4
   Sonstigeobtain the supernatant
5. 5
   Extraktionextracted 5 times with 5 mL of ethyl acetate

Vorschrift

100 mg of the lyophilized, surface expressing E. coli was suspended with 100 mg of phenylpyruvic acid, 7 mg of sodium formate and 1.32 mg of NAD in 10 mL of 0.1 M phosphate buffer (pH 7.0) solution and stirred for 48 hours for reaction. After completion of the reaction, the solution was centrifuged at 12,000 rpm for 3 minutes to remove cells and obtain the supernatant. The obtained solution was adjusted to pH 2.0, extracted 5 times with 5 mL of ethyl acetate and subjected to liquid chromatography (column: Chiralcel OD-H; flow rate: 0.5 mL/min; solvent: Hexane/iso-propanol/tri-fluoroacetic acid=80:20:1 (volume); detection: UV 210 nm). As a result, (R)-phenyl lactic acid with an optical purity of 90% or better was obtained.

Quelle