Reaktion #67593

Reaktionsgleichung

NaHCO3

5-(3,3-Dimethyl-but-1-ynyl)-3-[(4-methyl-cyclohexanecarbonyl)-pyrrolidin-3-yl-amino]-thiophene-2-carboxylic acid methyl ester

pyridine-2-carbonyl chloride HCl salt

lithium hydroxide

DIEA

→

title compound

5-(3,3-Dimethyl-but-1-ynyl)-3-{(4-methyl-cyclohexanecarbonyl)-[1-(pyridine-3-carbonyl)-pyrrolidin-3-yl]-amino}-thiophene-2-carboxylic acid

Lösungsmittel

brine

DCM

water

MeOH

Reaktionsbedingungen

Aufarbeitung

1. 1
   Extraktionthe crude product was extracted with ethyl acetate (2×20 mL)
2. 2
   EinengenThe combined organic layers were concentrated
3. 3
   workup.DISSOLUTIONthe crude material was dissolved in a 3:2:1 mixture of THF
4. 4
   Temperaturheated to 60 deg C
5. 5
   workup.WAITfor 2 hours
6. 6
   SonstigeThe residue was purified by HPLC (Gemini column, 35% acetonitrile
7. 7
   workup.ADDITIONwater, 2 min, 35-50% acetonitrile:water, 2 min, 50-100% acetonitrile: water 13 min, both solvents containing 0.1% trifluoroacetic acid)

Vorschrift

A mixture of 5-(3,3-Dimethyl-but-1-ynyl)-3-[(4-methyl-cyclohexanecarbonyl)-pyrrolidin-3-yl-amino]-thiophene-2-carboxylic acid methyl ester (74 mg, 0.158 mmol) and pyridine-2-carbonyl chloride HCl salt (56 mg, 0.31 mmol) in DCM (2 mL) was treated with DIEA (124 μL, 0.8 mmol). After 30 min, NaHCO3 (saturated aqueous solution, 4-8 mL) was added to the mixture, followed by brine (20 mL), and the crude product was extracted with ethyl acetate (2×20 mL). The combined organic layers were concentrated and the crude material was dissolved in a 3:2:1 mixture of THF:MeOH:water (5 mL), treated with lithium hydroxide (42 mg, 1 mmol,) and heated to 60 deg C. for 2 hours. The residue was purified by HPLC (Gemini column, 35% acetonitrile:water, 2 min, 35-50% acetonitrile:water, 2 min, 50-100% acetonitrile: water 13 min, both solvents containing 0.1% trifluoroacetic acid). This resulted in 37 mg of the title compound as its TFA salt: MS (m/z): 522.0 [M−H]+; HPLC retention time: 3.58 min (2-98% acetonitrile:water with 0.05% trifluoroacetic acid).

Quelle