Reaktion #64802

Reaktionsgleichung

water

Bis(trimethylsilyl)trifluoroacetamide

L-norleucine

2-trifluoromethyl-3-acetylthiopropionyl chloride

→

N-[2-[(Acetylthio)methyl]-3,3,3-trifluoro-1-oxopropyl]-L-norleucine

Lösungsmittel

ethyl acetate

hexane

methanol

acetonitrile

acetic acid

acetonitrile

Reaktionsbedingungen

Aufarbeitung

1. 1
   workup.DISSOLUTIONalmost all of the solid dissolved
2. 2
   Sonstigewas recooled to 0° C.
3. 3
   workup.STIRRINGAfter stirring at room temperature for 18 hours
4. 4
   Extraktionthe resulting mixture was extracted with ethyl acetate (500 mL)
5. 5
   workup.ADDITIONBrine was added
6. 6
   Sonstigeformed during this extraction
7. 7
   ExtraktionThe aqueous layer was extracted once more with ethyl acetate (100 mL)
8. 8
   Waschenthe combined organic layers were washed with brine
9. 9
   Trocknendried (MgSO4)
10. 10
    Einengenconcentrated
11. 11
    Sonstigeto afford an oily residue which
12. 12
    Sonstigewas pre-absorbed on Celite
13. 13
    Waschenwas eluted
14. 14
    EinengenThe pure less polar fractions were concentrated
15. 15
    Sonstigeto afford a white solid which
16. 16
    Sonstigewas recrystallized twice from ethyl acetate/hexane
17. 17
    SonstigeDrying under high vacuum at room temperature for 18 hours
18. 18
    Sonstigeafforded 1.95 g

Vorschrift

Bis(trimethylsilyl)trifluoroacetamide (16 mL., 60 mmol.) was added to a suspension of L-norleucine (2.62 g., 20 mmol.) in 40 mL of acetonitrile at 0° C. The suspension was allowed to warm to room temperature and was stirred for 2.5 hours, at which point, almost all of the solid dissolved. The reaction mixture was recooled to 0° C. and 2-trifluoromethyl-3-acetylthiopropionyl chloride (4.7 g., 20 mmol.) was added dropwise over 20 minutes as a solution in 10 mL of acetonitrile. After stirring at room temperature for 18 hours, the reaction was poured into 200 mL of water and the resulting mixture was extracted with ethyl acetate (500 mL). Brine was added to break up an emulsion which formed during this extraction. The aqueous layer was extracted once more with ethyl acetate (100 mL) and the combined organic layers were washed with brine, dried (MgSO4), and concentrated to afford an oily residue which was pre-absorbed on Celite. The crude product and Celite mixture was loaded onto an 8×30 cm silica gel column which was eluted as follows: 5L hexane:ethyl acetate:acetic acid (750:250:25), 2L hexane:ethyl acetate:acetic acid (700:300:25), 2L hexane:ethyl acetate:acetic acid (500:500:25). The pure less polar fractions were concentrated and co-evaporated from heptane to afford a white solid which was recrystallized twice from ethyl acetate/hexane. Drying under high vacuum at room temperature for 18 hours afforded 1.95 g. of the title isomer A compound as a white powder, m.p. 176°-178° C., TLC Rf =0.30 (ethyl acetate:hexane:acetic acid, 30:70:1). [α]D =-133.3° (c=0.6, methanol).

Quelle