Reaktion #62537

ord-206ec9e44fcb496288f80b4c23c1714c

Reaktionsgleichung

N[C@@H](Cc1c[nH]c2ccccc12)C(=O)O
L-tryptophan
Cc1ncc(COP(=O)(O)O)c(C=O)c1O
pyridoxalphosphate
O=P([O-])(O)O.O=P([O-])([O-])O.[K+].[K+].[K+]
K2HPO4 KH2PO4
O=C(O)C(Cl)(Cl)Cl
trichloroacetic acid
→
c1ccc2[nH]ccc2c1
Indole

Edukte

N[C@@H](Cc1c[nH]c2ccccc12)C(=O)O
L-tryptophan
Cc1ncc(COP(=O)(O)O)c(C=O)c1O
pyridoxalphosphate
O=P([O-])(O)O.O=P([O-])([O-])O.[K+].[K+].[K+]
K2HPO4 KH2PO4
—
aqueous solution
O=C(O)C(Cl)(Cl)Cl
trichloroacetic acid

Reagenzien

Keine

Lösungsmittel

—
reaction mixture

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    Sonstigethe reaction

Vorschrift

Measured in accordance with the method of Yamada et al. as follows. In 4.0ml of a reaction mixture containing 10 micromoles of L-tryptophan, 0.4 micromole of pyridoxalphosphate (PLP)), 200 micromoles of K2HPO4 -KH2PO4 (pH 8.0), the enzyme is incubated at 65° C. for 10 minutes. Then, 1.0 ml of a 30% aqueous solution of trichloroacetic acid (TCA) is added to stop the reaction. Indole formed in the reaction system is quantitatively determined by the method of E. McEvoy-Bowe [The ANALYST, vol. 88, pages 893-894 (1963)]. The activity is expressed in units in which 1 U is the amount in micromoles of indole formed during 1 minute.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US04761374uspto-grants-1988_08