Reaktion #574227

Reaktionsgleichung

glucose

potassium

magnesium sulfate

thiamine hydrochloride

biotin

DL-alanine

ammonium sulfate

nicotinamide

→

L-lysine

Reaktionsbedingungen

Aufarbeitung

1. 1
   workup.ADDITIONat pH 7.0, was charged in an amount of 300 ml
2. 2
   Sonstigeinto separate small sized glass jar fermenters

Vorschrift

An aqueous solution medium having a composition of 10% of glucose, 2% of ammonium sulfate, 0.1% of potassium primary phosphate, 0.04% of magnesium sulfate, 0.001% of ferrous sulfate, 200 μg/l of thiamine hydrochloride, 500 μg/l of biotin, 5 ml/dl of Aji-Eki®, 1 mg/dl of nicotinamide and 0.1% of DL-alanine, at pH 7.0, was charged in an amount of 300 ml into separate small sized glass jar fermenters. After sterilizing in a conventional manner, the various L-lysine-producing bacterial strains shown in Table 11, which had been previously grown in bouillon slants at 30° C. for 48 hours, were inoculated thereon. Then, incubation was carried out at 31.5° C. for 48 hours at 1200 rpm at an aeration rate of 1/2 volume per minute, while keeping the pH at 7.0 by the addition of ammonia gas. After completion of the fermentation, the yield of L-lysine produced and accumulated in the solution based on glucose was determined and is shown in Table 11.

Quelle