Reaktion #558522

Reaktionsgleichung

diethyl 2-benzyloxycarbonylamino-2-ethoxycarbonylsuccinate

→

(R)-1-ethyl hydrogen 3-benzyloxycarbonylamino-3-ethoxycarbonylsuccinate

Ausbeute 52.0%

Lösungsmittel

phosphate

ethanol

Reaktionsbedingungen

Aufarbeitung

1. 1
   Sonstige5 mg of rabbit liver esterase produced by Sigma (80 kU/g, lyophilized product, product number: E0887)

Vorschrift

5 mg of rabbit liver esterase produced by Sigma (80 kU/g, lyophilized product, product number: E0887) was dissolved in a 0.05 mol/L phosphate buffer adjusted to pH 6.5 (0.4 mL), and the solution was added with a solution of diethyl 2-benzyloxycarbonylamino-2-ethoxycarbonylsuccinate (5 mg) dissolved in ethanol (0.05 mL). The mixture was stirred at 30° C. for 16 hours to obtain (R)-1-ethyl hydrogen 3-benzyloxycarbonylamino-3-ethoxycarbonylsuccinate at a yield of 52% and optical purity of 94.6% ee.

Quelle