Reaktion #554589

ord-6103723f4aa54a28b7944172c9a12d17

Reaktionsgleichung

[Na+].[OH-]
sodium hydroxide
O=P(O)(O)O
phosphoric acid
O=P(O)(O)O[C@H]1[C@H](OP(=O)(O)O)[C@@H](OP(=O)(O)O)[C@H](OP(=O)(O)O)[C@@H](OP(=O)(O)O)[C@H]1OP(=O)(O)O
phytic acid
→
O=P(O)(O)OC1C(OP(=O)(O)O)C(OP(=O)(O)O)C(OP(=O)(O)O)C(OP(=O)(O)O)C1OP(=O)(O)O
phytate

Edukte

[Na+].[OH-]
sodium hydroxide
O=P(O)(O)O
phosphoric acid
O=P(O)(O)O[C@H]1[C@H](OP(=O)(O)O)[C@@H](OP(=O)(O)O)[C@H](OP(=O)(O)O)[C@@H](OP(=O)(O)O)[C@H]1OP(=O)(O)O
phytic acid

Reagenzien

Keine

Lösungsmittel

O
water

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    Extraktionoil therein was extracted
  2. 2
    Sonstigeseparated
  3. 3
    Sonstigeremoved
  4. 4
    Sonstigeto obtain low
  5. 5
    ExtraktionNext, it was extracted
  6. 6
    Sonstigeto separate bean curd refuse and insolubles
  7. 7
    Sonstigeto obtain
  8. 8
    Sonstigeto obtain
  9. 9
    Sonstigean insoluble fraction (acid precipitated curd)
  10. 10
    workup.ADDITIONWater was added so that the acid
  11. 11
    Sonstigeprecipitated curd
  12. 12
    Sonstigeto obtain an acid
  13. 13
    Sonstigeprecipitated curd slurry
  14. 14
    Temperaturwas heated to 40° C
  15. 15
    workup.ADDITIONTo this solution (phytic acid content 1.96% by mass; solid content, TCA solubilization ratio 4.6%) was added phytase (manufactured by NOVO, Inc.) of equivalent to 8 units per solid content
  16. 16
    workup.WAITto act for 30 minutes
  17. 17
    SonstigeAfter the reaction
  18. 18
    Temperaturheat treatment
  19. 19
    workup.WAITwas performed at 120° C. for 15 seconds
  20. 20
    SonstigeThis was then spray dried

Vorschrift

Soybeans were pressurized, and then oil therein was extracted, separated, and removed using n-hexane as an extraction solvent to obtain low denatured defatted soybeans. To 5 kg of the obtained low denatured defatted soybeans (nitrogen solubility index (NSI) 91) was added 35 kg of water, and the pH was adjusted to 7 using diluted sodium hydroxide solution. Next, it was extracted while stirring for one hour at room temperature, and then centrifuged at 4000 G to separate bean curd refuse and insolubles, to obtain defatted soybean milk. The pH of this defatted soybean milk was adjusted to 4.5 using phosphoric acid, and then centrifuged using a continuous centrifugal separator (decanter) at 2000 G, to obtain an insoluble fraction (acid precipitated curd) and a soluble fraction (whey). Water was added so that the acid precipitated curd became 10% by mass of solid content, to obtain an acid precipitated curd slurry. After the pH of this was adjusted to 4.0 using phosphoric acid, it was heated to 40° C. To this solution (phytic acid content 1.96% by mass; solid content, TCA solubilization ratio 4.6%) was added phytase (manufactured by NOVO, Inc.) of equivalent to 8 units per solid content, allowing the enzymes to act for 30 minutes. After the reaction, the pH of this enzyme-applied product (phytic acid content 0.04% by mass; solid content, TCA solubilization ratio substantially unchanged) was adjusted to 3.5, and heat treatment was performed at 120° C. for 15 seconds, using a continuous direct heat treatment sterilizer. This was then spray dried, to obtain 1.5 kg of low phytate soybean protein powder. The dissolution ratio of this protein (pH 3.5) was 95%.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US08623443B2uspto-grants-2014_01