Reaktion #546917

Reaktionsgleichung

NAD+

phosphate

NaOH

→

NADH

Ausbeute 24.2%

Reaktionsbedingungen

Aufarbeitung

1. 1
   SonstigeThe Fisher-Porter bottle was evacuated
2. 2
   workup.ADDITIONrefilled with argon three times
3. 3
   SonstigeThe Fisher-Porter bottle was evacuated
4. 4
   workup.ADDITIONfilled with 70 psi of H2
5. 5
   SonstigeThe volatiles were removed under reduced pressure
6. 6
   workup.DISSOLUTIONa sample dissolved in D2O To
7. 7
   workup.ADDITIONthis was added 20 μl of trimethylsilylpropionate standard solution

Vorschrift

NAD+ (34.0 mg, 38.0 μmol) was placed in 2 mL of a 0.1 M phosphate buffer. The pH of this solution was adjusted to 8.1 with NaOH and then placed in an 80 mL Fisher-Porter bottle. The Fisher-Porter bottle was evacuated and refilled with argon three times then [Cl2Ru(TPPTS)2]2 (6.9 mg, 2.6 μmol) was added under a flow of argon. The Fisher-Porter bottle was evacuated and filled with 70 psi of H2 and stirred to 23° C. for 17.0 hr. The volatiles were removed under reduced pressure and a sample dissolved in D2O To this was added 20 μl of trimethylsilylpropionate standard solution. 1H NMR integration shows that 9.2 μmole of NADH have been produced under these conditions.

Quelle