Reaktion #53249

Reaktionsgleichung

ethylenediamine

diisopropylethylamine

cholesterol chloroformate

cholesterol chloroformate

ethylenediamine

→

Cholesterol ethylenediamine

Lösungsmittel

DCM

dichloromethane

Reaktionsbedingungen

Aufarbeitung

1. 1
   workup.STIRRINGstir
2. 2
   Sonstigeorganic solvent was roto-evaporated away
3. 3
   workup.ADDITIONApproximately 400 mL of acetonitrile was added
4. 4
   Sonstigeto precipitate the crude product
5. 5
   FiltrationThe solid was then collected by filtration onto a Whatman #5
6. 6
   Filtrationfilter apparatus
7. 7
   Waschenrinsed several times with acetonitrile
8. 8
   Sonstigeair-dried
9. 9
   workup.DISSOLUTIONThe crude solid was dissolved in a minimal volume of chloroform
10. 10
    Sonstigechromatographically purified

Vorschrift

Solid cholesterol chloroformate (10 mmol) was dissolved in 100 mL of dichloromethane (DCM). In a separate flask 0.5 mol ethylenediamine was diluted with 200 mL of DCM and 20 mmol diisopropylethylamine. While stirring, the cholesterol chloroformate solution was drop-wise added to the ethylenediamine solution over a 30 minute period at room temperature (R.T.). Following an overnight R.T. stir, organic solvent was roto-evaporated away using medium vacuum until a minimal sample volume remained. Approximately 400 mL of acetonitrile was added to precipitate the crude product. The solid was then collected by filtration onto a Whatman #5 filter apparatus, rinsed several times with acetonitrile and then air-dried. The crude solid was dissolved in a minimal volume of chloroform and chromatographically purified using a silica column. The purified product presented a single TLC spot (4:1 chloroform/methanol), and a single peak (parent ion M+H=474) was identified by positive mode electrospray.

Quelle