Reaktion #51229

Reaktionsgleichung

sodium hydroxide

N-benzyloxycarbonyl-trans-4-[(6-nitro-1-indolinyl)carbonyl]cyclohexylamine

→

N-benzyloxycarbonyl-trans-4-[(6-amino-1-indolinyl)carbonyl]cyclohexylamine

Ausbeute 83.5%

Lösungsmittel

chloroform

Reaktionsbedingungen

Aufarbeitung

1. 1
   Sonstigeobtained in the same manner as in Reference Example 13-1 before deprotection), and the mixture
2. 2
   Temperaturwas refluxed under argon atmosphere for 4.5 hours
3. 3
   Trocknendried over anhydrous magnesium sulfate
4. 4
   Sonstigethe insolubles were removed by filtration
5. 5
   EinengenThe filtrate was concentrated under reduced pressure
6. 6
   Sonstigethe resulting residue was purified by silica gel column chromatography (solvent: chloroform-ethyl acetate (2:1))

Vorschrift

17.33 g of stannous chloride was added to a hydrated ethanol (120 ml of ethanol+1.2 ml of water) suspension containing 6.08 g of N-benzyloxycarbonyl-trans-4-[(6-nitro-1-indolinyl)carbonyl]cyclohexylamine (the compound obtained in the same manner as in Reference Example 13-1 before deprotection), and the mixture was refluxed under argon atmosphere for 4.5 hours. An aqueous 10% sodium hydroxide solution was added to the reaction mixture to adjust pH of the mixture to pH 9 to 10, the mixture was diluted with 300 ml of chloroform and dried over anhydrous magnesium sulfate, and then, the insolubles were removed by filtration. The filtrate was concentrated under reduced pressure, and the resulting residue was purified by silica gel column chromatography (solvent: chloroform-ethyl acetate (2:1)) to obtain 4.72 g of N-benzyloxycarbonyl-trans-4-[(6-amino-1-indolinyl)carbonyl]cyclohexylamine.

Quelle