Reaktion #506158

ord-c2cff203c9cd412ba440733db348b2bd

Reaktionsgleichung

O=S(=O)([O-])[O-].[NH4+].[NH4+]
ammonium sulfate
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
O.O.O.O.O.O.O.O=S(=O)([O-])[O-].[Mg+2]
magnesium sulfate heptahydrate
O=P([O-])(O)O.[K+]
potassium dihydrogen phosphate
Cc1ncc(C[n+]2csc(CCO)c2C)c(N)n1.Cl.[Cl-]
thiamine hydrochloride
NC(=O)c1cccnc1
nicotine amide
O=C(O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21
biotin
→
Nc1cc(O)cc(C(=O)O)c1
AHBA

Edukte

—
Example 3 ( 2 )
O=S(=O)([O-])[O-].[NH4+].[NH4+]
ammonium sulfate
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
O.O.O.O.O.O.O.O=S(=O)([O-])[O-].[Mg+2]
magnesium sulfate heptahydrate
O=P([O-])(O)O.[K+]
potassium dihydrogen phosphate
Cc1ncc(C[n+]2csc(CCO)c2C)c(N)n1.Cl.[Cl-]
thiamine hydrochloride
NC(=O)c1cccnc1
nicotine amide
—
( 3 )
O=C(O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21
biotin

Reagenzien

O.O.O.O.O.O=S(=O)([O-])[O-].[Mn+2]
manganese sulfate pentahydrate
O.O.O.O.O.O.O.O=S(=O)([O-])[O-].[Fe+2]
iron sulfate heptahydrate

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    Einengenconcentrate (hydrolyzed soy protein, as total nitrogen content) and 50 g of calcium carbonate
  2. 2
    workup.ADDITIONwas added at a final concentration of 25 mg/L and chloramphenicol
  3. 3
    workup.ADDITIONwas added at a final concentration of 5.0 mg/L) at 30° C. for 71 hours at 120 rpm
  4. 4
    SonstigeAs a result, glucose was completely consumed in all of the experiments
  5. 5
    workup.ADDITIONglutamicum AJ110135 having introduced pVC7 as the control experiment

Vorschrift

The C. glutamicum AJ110135 having an enhanced AKfbr gene and the C. glutamicum AJ110135 having an enhanced PC gene selected in Example 3 (2) and (3), respectively were cultured in the flask evaluation medium (100 g of glucose, 1 g of magnesium sulfate heptahydrate, 55 g of ammonium sulfate, 1 g of potassium dihydrogen phosphate, 0.01 g of iron sulfate heptahydrate, 0.01 g of manganese sulfate pentahydrate, 2 mg of thiamine hydrochloride, 0.5 mg of biotin, 5 mg of nicotine amide, 1.05 g of soy concentrate (hydrolyzed soy protein, as total nitrogen content) and 50 g of calcium carbonate were adjusted in 1 L of water to pH 7.2, and kanamycin was added at a final concentration of 25 mg/L and chloramphenicol was added at a final concentration of 5.0 mg/L) at 30° C. for 71 hours at 120 rpm. As the control experiment, the C. glutamicum AJ110135 with the pVC7 was cultured in the above flask evaluation medium with kanamycin at a final concentration of 25 mg/L for 71 hours. As a result, glucose was completely consumed in all of the experiments. 1.0 g/L of 3,4-AHBA was accumulated in the culture of C. glutamicum AJ110135 having the enhanced AKfbr gene, and 0.6 g/L of 3,4-AHBA was accumulated in the culture of C. glutamicum AJ110135 having the enhanced PC gene (Table 2). Meanwhile, 0.5 g/L of 3,4-AHBA was accumulated in the culture of C. glutamicum AJ110135 having introduced pVC7 as the control experiment. From the above results, the ability to form AHBA was improved in C. glutamicum AJ110135 having the enhanced AKfbr gene and C. glutamicum AJ110135 having the enhanced PC gene compared with C. glutamicum AJ110135 as the control.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US08093346B2uspto-grants-2012_01