Reaktion #498289

ord-0c0a8a6d4ff944e5b318df6c0b5ebdbd

Reaktionsgleichung

NCC(=O)O
glycine
O=[N+]([O-])c1ccc(O[C@@H]2O[C@H](CO)[C@@H](O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)cc1
p-nitrophenyl-β-D-cellobioside
O=C1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O
gluconolactone
CC(=O)O.[Na]
sodium acetic acid
→
O=[N+]([O-])c1ccc(O)cc1
p-nitrophenol

Edukte

—
solution
NCC(=O)O
glycine
O=[N+]([O-])c1ccc(O[C@@H]2O[C@H](CO)[C@@H](O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)cc1
p-nitrophenyl-β-D-cellobioside
O=C1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O
gluconolactone
CC(=O)O.[Na]
sodium acetic acid

Reagenzien

Keine

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    Sonstigean incubation of 15 minutes
  2. 2
    Sonstigeat 30, 40, 50, 60 or 70° C.

Vorschrift

To determine the influence of the temperature on the enzyme activity 25 μl enzyme solution was added to 225 μl of a solution containing 0.1% p-nitrophenyl-β-D-cellobioside and 25 mM gluconolactone in a 50 mM sodium acetic acid buffer at pH 5.0. Following an incubation of 15 minutes at 30, 40, 50, 60 or 70° C. the enzyme activity was stopped by adding 750 μl of a 0.5 M glycine buffer pH 9.0. The concentration of p-nitrophenol formed was determined spectrophotometrically at 400 nm.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US06558937B1uspto-grants-2003_05