Reaktion #483136

Reaktionsgleichung

bicarbonate

4-t-butoxycarbonyl aminobutyric acid

4-methylmorpholine

6-Amino-3-(4-pyridyl)-2-(4-fluorophenyl)-7-aza-indole

Isobutyl chloroformate

→

6-(4′-t-butoxycarbonylamino-1′-oxo-butylamino)-3-(4-pyridyl)-2-(4-fluorophenyl)-7-aza-indole

Lösungsmittel

THF

THF

Reaktionsbedingungen

Aufarbeitung

1. 1
   workup.WAITAfter 16 h at 23° C.
2. 2
   Extraktionextracted with ethyl acetate (2×100 mL)
3. 3
   Waschenwashed with brine (1×100 mL)
4. 4
   Trocknendried (Na2SO4)
5. 5
   EinengenAfter concentration in vacuo
6. 6
   Sonstigethe residue was purified by application to two preparative chromatography plates (silica gel 2 mm thickness, 100% ethyl acetate)

Vorschrift

General Procedure for mixed anhydride coupling—Isobutyl chloroformate (32 ml, 0.24 mmol) was added dropwise to a −20-30° C. solution of 4-t-butoxycarbonyl aminobutyric acid (19) (50.1 mg, 0.247 mmol), 4-methylmorpholine (124 ml, 1.23 mmol), and THF (2 mL). After 20 min at −20-30° C., 6-Amino-3-(4-pyridyl)-2-(4-fluorophenyl)-7-aza-indole (17) (75 mg, 0.24 mmol) and THF (3 mL) was added in one portion. The reaction was allowed to warm to 23° C. After 16 h at 23° C., the reaction was poured into saturated bicarbonate (80 mL), extracted with ethyl acetate (2×100 mL), washed with brine (1×100 mL), and dried (Na2SO4). After concentration in vacuo, the residue was purified by application to two preparative chromatography plates (silica gel 2 mm thickness, 100% ethyl acetate) to afford 6-(4′-t-butoxycarbonylamino-1′-oxo-butylamino)-3-(4-pyridyl)-2-(4-fluorophenyl)-7-aza-indole (20) as a solid: Mass Spectrum (CI) 489 (MH+). Ethyl chloroformate can be used in place of isobutyl chloroformate in this process.

Quelle