Reaktion #469425
ord-118f7650165245aeb4a18dc0505d1c24
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Microbial conversions of unsaturated fatty acids have been widely exploited to produce new, value-added products. Hou, (C. T Hou, “Microbial Oxidation of Unsaturated Fatty Acids”, Advances in Applied Microbiology Vol. 41, pp. 1-23, 1995) recently reviewed the work on some biological oxidation systems. Wallen et al., (L. L. Wallen et al., “The Microbiological Production of 10-Hydroxystearic Acid from Oleic acid”, Arch. Biochem. Biophys. 99:249-253, 1962) reported the first bioconversion of oleic acid to 10-hydroxystearic acid by a Pseudomonad. The bioconversion of fatty acids to produce mono-, di-, and tri-hydroxy unsaturated fatty acids has also been found (A. C. Lanser et al., “Production of 15-, 16- and 17-Hydroxy-9-Octadecenoic Acid from Oleic Acid with Bacillus pumilus”, J. Am. Oil Chem. Soc. 69:363-366, 1992), (C. T. Hou et al., “A Novel Compound, 7,10-Dihydroxy-8(E)-Octadecenoic Acid from Oleic Acid by Bioconversion”, J. Am. Oil Chem. Soc. 68:99-101, 1991), (C. T. Hou et al., “Production of a New Compound, 7,10-Dihydroxy-8(E)-Octadecenoic Acid from Oleic Acid by Pseudomonas sp. PR3”, J. Indust. Microbial. 7:123-130, 1991) and (C. T. Hou et al., “A Novel Compound, 12,13,17-Trihydroxy-9(Z)-Octadecenoic Acid, from Linoleic Acid by a New Microbial Isolate Clavibacter sp. ALA2”, J. Am. Oil Chem. Soc. 73:1359-1362, 1996). The production of a novel compound, 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) from oleic acid by strain PR3 has been described most extensively. Under optimal conditions, the yield of bioconversion is greater than 60%, (C. T. Hou et al., “Production of a New Compound, 7,10-Dihydroxy-8(E)-Octadecenoic Acid from Oleic Acid by Pseudomonas sp. PR3”, J. Indust. Microbiol. 7:123-130, 1991). PR3 is a strain of Pseudomonas aeruginosa, and its DOD production is inversely correlated with the accumulation of phenazine 1-carboxylic acid (PCA), (C. T. Hou et al., “Identification of NRRL Strain B-18602 (PR3) as Pseudomonas Aeruginosa and Effect of Phenazine-1-Carboxylic Acid Formation on 7,10-Dihydroxy-8(E)-Octadecenoic Acid Accumulation”, World J. Microbiol. Biotechnol. 9:570-573, 1993). The production of DOD and PCA by strain PR3, however, was not consistent, and studies were conducted to stabilize and maximize cultures for the bioconversion of oleic acid.