Reaktion #461925

ord-2aee406fd45a4496adfbf3ee07d532d9

Reaktionsgleichung

NCCCCCC(=O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21
Biotinylpentylamine
[O-]B([O-])[O-]
borate
→
O=C(O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21
biotin

Edukte

—
isothiocyanatobenzyl-diethylenetriamine-pentaacetic acid
NCCCCCC(=O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21
Biotinylpentylamine
[O-]B([O-])[O-]
borate
—
biotinylpentylamino-thioureaylbenzyl-DTPA

Reagenzien

Keine

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    SonstigeThe biotin-DTPA is purified on a C-18 reverse-phase HPLC column
  2. 2
    SonstigeThe acetonitrile is removed under reduced pressure on a rotary evaporator
  3. 3
    Sonstigethe water by lyophilization to give a white powdery product

Vorschrift

Biotinylpentylamine (0.001 mol) and isothiocyanatobenzyl-diethylenetriamine-pentaacetic acid (ITC-DTPA, 0.001 mol) are mixed together in borate buffer, 0.1M, pH 8, and stirred for 3 hours at room temperature. The biotin-DTPA is purified on a C-18 reverse-phase HPLC column run in an acetonitrile/water gradient. The acetonitrile is removed under reduced pressure on a rotary evaporator and the water by lyophilization to give a white powdery product. The structure, biotinylpentylamino-thioureaylbenzyl-DTPA (biotin-DTPA) is confirmed by mass spectral and micro analysis.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US05482698uspto-grants-1996_01