Reaktion #460917

Reaktionsgleichung

[3S-(3α,4β,5α)]-4-amino-1-[tetrahydro-4,5-bis(hydroxymethyl)-3-furanyl]-2(1H)-pyrimidinone

iodic acid

iodine

→

title compound

Ausbeute 73.4%

[3S-(3α,4β,5 α)]-4-Amino-5-iodo-1-[tetrahydro-4,5-bis(hydroxymethyl)-3-furanyl]-2(1H)-pyrimidinone

Ausbeute 73.4%

Lösungsmittel

water

acetic acid

carbon tetrachloride

Reaktionsbedingungen

Aufarbeitung

1. 1
   Einengenit was then concentrated in vacuo
2. 2
   Sonstigeto yield a dark residue
3. 3
   SonstigeThe excess iodine was removed from the residue
4. 4
   Sonstigeby azeotroping several times with methanol
5. 5
   workup.DISSOLUTIONThe crude material was dissolved in water (3 ml)
6. 6
   SonstigeThe aqueous mixture was purified on a CHP-20P resin column (Mitsubishi Chemical Co., 75-150 μ, 20 ml)
7. 7
   Wascheneluting with water (150 ml)
8. 8
   Sonstigethe volatiles were removed in vacuo
9. 9
   workup.DISSOLUTIONthe residue dissolved in water

Vorschrift

To a solution of [3S-(3α,4β,5α)]-4-amino-1-[tetrahydro-4,5-bis(hydroxymethyl)-3-furanyl]-2(1H)-pyrimidinone (96.5 mg, 0.4 mmol) in water (160 ml), acetic acid (320 μl) and carbon tetrachloride (80 μl) was added iodic acid (36 mg, 0.204 mmol) and iodine (60 mg, 0.236 mmol). The resulting mixutre was heated at 50° C. for 2 hours and it was then concentrated in vacuo to yield a dark residue. The excess iodine was removed from the residue by azeotroping several times with methanol. The crude material was dissolved in water (3 ml), and the pH was adjusted to 7 with 1 N sodium hydroxide. The aqueous mixture was purified on a CHP-20P resin column (Mitsubishi Chemical Co., 75-150 μ, 20 ml) eluting with water (150 ml) and then 5% acetonitrile/water (300 ml). The appropriate fractions were combined, the volatiles were removed in vacuo, and the residue dissolved in water and lyophilized to give the title compound (55 mg) as a colorless solid. Proton NMR (270 MHz, DMSO-d6) δ:8.16 (s, 1H), 7.65 (brs, 1H), 6.50 (brs, 1H), 4.92 (t, J=5.2 Hz, 1H), 4.87 (m, 1H), 4.80 (t, J=5.2 Hz, 1H), 3.60-3.90 (m, 4H), 3.51 (m, 3H), 2.24 (m, 1H); M.P.=212°-216° C. (dec.).

Quelle