Reaktion #450468

ord-fd1c569cce7d430b8dd60d7fe1707974

Reaktionsgleichung

Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
ATP
OC[C@H]1O[C@@H](n2cnc3c(O)ncnc32)[C@H](O)[C@@H]1O
inosine
O=S(=O)([O-])[O-].[Mg+2]
magnesium sulfate
→
O=c1nc[nH]c2c1ncn2[C@@H]1O[C@H](COP(=O)(O)O)[C@@H](O)[C@H]1O
5'-IMP

Edukte

—
51
—
DNA
—
Example 2 ( 3 )
Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
ATP
OC[C@H]1O[C@@H](n2cnc3c(O)ncnc32)[C@H](O)[C@@H]1O
inosine
O=S(=O)([O-])[O-].[Mg+2]
magnesium sulfate

Reagenzien

Keine

Lösungsmittel

—
Tris-hydrochloride
Cc1ccccc1C
Xylene

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    workup.WAITto stand at 30° C. for an hour

Vorschrift

Escherichia coli cells containing the recombinant DNA collected in Example 2 (3) were suspended (cell concentration was 100 g wet cell weight/ml) in 100 mM Tris-hydrochloride buffer (pH 8.0) containing 10 mM ATP, 10 mM inosine and 5 mM magnesium sulfate. Xylene was added to the suspension in a concentration of 10 ml/l. After thoroughly stirring, the mixture was allowed to stand at 30° C. for an hour. The reaction solution was analyzed by HPLC and an amount of 51 -IMP in the reaction solution was quantitatively determined. Almost all transformants did not produce 5'-IMP but 5'-IMP-producing bacteria were obtained in a proportion of 1 out of 50,000 samples, The thus obtained 5'-IMP-producing bacteria are transformants carrying a recombinant DNA containing inosine guanosine-kinase gene.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US05874272uspto-grants-1999_02