Reaktion #450343

Reaktionsgleichung

(6-aminohexanoyl)-4-amino-methylsalicylhydroxamic acid

HCl

→

Sepharose

Salicylhydroxamic Acid

Lösungsmittel

NaHCO3

Reaktionsbedingungen

Aufarbeitung

1. 1
   Waschenwashed CNBr
2. 2
   SonstigeSepharose 4B (Pharmacia) overnight
3. 3
   Sonstigeat room temperature
4. 4
   SonstigeAfter the coupling reaction, 2 mL of 0.5M Tris, pH 8.5
5. 5
   workup.ADDITIONwere added
6. 6
   workup.ADDITIONthe gel slurry mixed at room temperature for 1 hour
7. 7
   Waschenwashed with water, 0.5M NaCl, and water again
8. 8
   Sonstigestored at 4° C.

Vorschrift

SHA-Sepharose 4B was prepared by mixing 130 mg of (6-aminohexanoyl)-4-amino-methylsalicylhydroxamic acid (SHA--Z--NH2), dissolved in 30 mL 0.2M NaHCO3, with 6.5 g HCl washed CNBr activated Sepharose 4B (Pharmacia) overnight at room temperature. After the coupling reaction, 2 mL of 0.5M Tris, pH 8.5 were added and the gel slurry mixed at room temperature for 1 hour, and washed with water, 0.5M NaCl, and water again. The resulting SHA-Sepharose 4B was suspended in 30 mL of 20% ethanol, and stored at 4° C.

Quelle