Reaktion #431818

ord-ac0be50a77a4496f8aaaf30ce0cdb399

Reaktionsgleichung

N[C@@H](CCC(=O)O)C(=O)O
Glu
NCC(=O)O
Gly
CC[C@H](C)[C@H](NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CCCCN)NC(=O)CNC(=O)[C@@H]1CCC(=O)N1)C(=O)N[C@@H](CC(C)C)C(=O)O
Xenopsin
→
NCCCC[C@H](N)C(=O)O
Lys
N=C(N)NCCC[C@H](N)C(=O)O
Arg
N=C(N)NCCC[C@H](NC(=O)[C@H](CCCCN)NC(=O)CNC(=O)[C@@H]1CCC(=O)N1)C(=O)O
pGlu-Gly-Lys-Arg

Edukte

—
amino acid
N[C@@H](CCC(=O)O)C(=O)O
Glu
NCC(=O)O
Gly
—
amino acid
CC[C@H](C)[C@H](NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CCCCN)NC(=O)CNC(=O)[C@@H]1CCC(=O)N1)C(=O)N[C@@H](CC(C)C)C(=O)O
Xenopsin

Reagenzien

Keine

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    Sonstigethe reaction for 20 hours
  2. 2
    Sonstigepeaks formed by chromatography

Vorschrift

200 mU of Benz-I was reacted with 50 nmol of Xenopsin (pGlu-Gly-Lys-Arg-Pro-Trp-Ile-Leu). After carrying out the reaction for 20 hours, peaks formed by chromatography were fractionated and the amino acid composition of each fraction was analyzed. As a result, it was found that a peak of a fragment assumed to have an amino acid composition of Glu: 0.993; Gly: 1.22; Lys: 0.674 and Arg: 1.11, namely, pGlu-Gly-Lys-Arg was obtained.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US05053333uspto-grants-1991_10