Reaktion #42643

ord-0abf829f876f400f8a0f7cb628a03d6a

Reaktionsgleichung

OC[C@H]1O[C@@](CO)(O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@@H](O)[C@@H]1O
sucrose
CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3OC4(O[C@H]23)O[C@H]([C@@H](N)CO)[C@H](O)[C@@H](O)[C@H]4O)[C@@H]1O
hygromycin B
→
CN[C@@H]1C[C@H](N)[C@@H](O)[C@H](O[C@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@@]4(O[C@@H]23)O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)[C@H]1O
hygromycin

Edukte

OC[C@H]1O[C@@](CO)(O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@@H](O)[C@@H]1O
sucrose
CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3OC4(O[C@H]23)O[C@H]([C@@H](N)CO)[C@H](O)[C@@H](O)[C@H]4O)[C@@H]1O
hygromycin B

Reagenzien

Keine

Lösungsmittel

—
obtained solution

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    workup.ADDITIONwas added to the resultant

Vorschrift

After completion of the electroshock, 1 ml of Vogel's minimal medium that contained 1.2% sucrose was added to the resultant, and the obtained mixture was then incubated at 30° C. for 2 hours. 200 μl of the obtained solution was applied to and expanded on an agar medium that contained hygromycin B (500 μg/ml). A hygromycin-resistance colony was isolated, and it was then confirmed by PCR whether or not substitution took place in the target locus. In addition, it was also confirmed by the Southern blot method whether or not it contained redundant Hygr gene copies.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US07732208B2uspto-grants-2010_06