Reaktion #416850

Reaktionsgleichung

p-nitrobenzyl 2-(4-[2-hydroxyethyl]-1,2,3-triazol-1-yl)-carbapen-2-em-3-carboxylate

4-morpholinepropanesulfonic acid

→

2-(4-[2-hydroxyethyl]-1,2,3-triazol-1-yl)-carbapen-2-em-3-carboxylic acid

Lösungsmittel

dioxane

water

ethanol

Reaktionsbedingungen

Aufarbeitung

1. 1
   Waschenthe pellet washed with deionized water (3×0.5 ml)
2. 2
   ExtraktionThe combined supernate and washes were extracted with ethyl acetate (3×0.5 ml)
3. 3
   Einengenconcentrated under vacuum

Vorschrift

A solution of p-nitrobenzyl 2-(4-[2-hydroxyethyl]-1,2,3-triazol-1-yl)-carbapen-2-em-3-carboxylate (3.5 mg, 8.8 micromol) in dioxane (300 microliter), deionized water (180 microliter), absolute ethanol (24 microliter), and pH 7.0 4-morpholinepropanesulfonic acid buffer (60 microliter of 0.5M) was shaken with 10% (w/w) palladium on charcoal (7.0 mg) under hydrogen (50 psig) at ambient temperature. After 40 min. the mixture was centrifuged and the pellet washed with deionized water (3×0.5 ml). The combined supernate and washes were extracted with ethyl acetate (3×0.5 ml) and concentrated under vacuum to provide 0.5 ml of a solution of 2-(4-[2-hydroxyethyl]-1,2,3-triazol-1-yl)-carbapen-2-em-3-carboxylic acid, UV λmax 299 nm which quenched upon addition of hydroxylamine hydrochloride and dipotassium hydrogen phosphate.

Quelle