Reaktion #354364

ord-4b6ddb4097a74bda95fdc984c65e90cf

Reaktionsgleichung

O=C(Cc1ccccc1)Nc1ccc([N+](=O)[O-])c(C(=O)O)c1
NIPAB
CC1(C)S[C@@H]2[C@H](NC(=O)Cc3ccccc3)C(=O)N2[C@H]1C(=O)[O-].[K+]
potassium penicillin G
→
CC1(C)S[C@@H]2[C@H](N)C(=O)N2[C@H]1C(=O)O
6-APA

Edukte

O=C(Cc1ccccc1)Nc1ccc([N+](=O)[O-])c(C(=O)O)c1
NIPAB
CC1(C)S[C@@H]2[C@H](NC(=O)Cc3ccccc3)C(=O)N2[C@H]1C(=O)[O-].[K+]
potassium penicillin G

Reagenzien

Keine

Lösungsmittel

O
water

Reaktionsbedingungen

Temperatur
38°CELSIUS
Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    Sonstige330 g of carrier-bound penicillinacylase produced
  2. 2
    SonstigeThe penicillin G is completely split to 6-APA and phenylacetic acid over the course of 2 hours

Vorschrift

330 g of carrier-bound penicillinacylase produced according to Example 1, having an enzymatic activity of 3,410 U (NIPAB test) and 129 g of potassium penicillin G are successively added to 2,000 ml of water and stirred at 38°C. and pH 7.8 as described in Example 14. The penicillin G is completely split to 6-APA and phenylacetic acid over the course of 2 hours. The 6-APA is isolated as described in Example 14. The carrierbound penicillinacylase is employed 20 times in succession. Even after the twentieth splitting, the reaction time does not have to be extended to achieve complete splitting.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US03941756uspto-grants-1976_03