Reaktion #345406

Reaktionsgleichung

glycine

furaldehyde

→

α-Amino-β-hydroxy-β-(2-furyl)propionic acid

Reaktionsbedingungen

Aufarbeitung

1. 1
   SonstigeThe substrate solution was prepared
2. 2
   workup.ADDITIONWhen 360 μL of this was added to the enzyme solution (0.2 mg SHMT in 40 μL)

Vorschrift

The substrate solution was prepared by dissolving glycine (14.0 mg, 0.186 mmol) and furaldehyde (15.5 μL, 0.187 mmol) in buffer (10.0 mL). When 360 μL of this was added to the enzyme solution (0.2 mg SHMT in 40 μL), the concentration of each substrate was 17 mM.

Quelle